| Component | Vendor | Catalog number | Final Concentration |
|---|---|---|---|
| DMEM/F2 | Gibco | 11320-033 | - |
| Horse Serum | Invitrogen | 16050-122 | 5% |
| EGF | Peprotech | AF-100-15 | 20 ng/ml |
| Hydrocortisone | Sigma | H0888 | 0.5 mg/ml |
| Cholera toxin | Sigma | C8052 | 100 ng/ml |
| Insulin | Sigma | I9278 | 10 ug/ml |
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