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. Author manuscript; available in PMC: 2022 Mar 2.
Published in final edited form as: Cell Metab. 2021 Feb 8;33(3):649–665.e8. doi: 10.1016/j.cmet.2021.01.014
Component Vendor Catalog number Final Concentration
Imaging base-DMEM/F12-noAA Gibco Custom; equivalent to Gibco 11320-033 lacking glucose, glutamine, amino acids, pyruvate, riboflavin, folic acid, and phenol red -
D-glucose Fisher D16 17 mM
BSA Invitrogen 16050-122 0.1% w/v
Hydrocortisone Sigma H0888 0.5 mg/ml
Cholera toxin Sigma C8052 100 ng/ml
Penicillin-Streptomycin Gibco 15140122 100 U/ml
EGF Peprotech AF-100-15 20 ng/ml
Insulin Sigma I9278 10 ug/ml