Table 1.
Complement Markers and Mediators
| Complement Component | Sample | Transf. | Outl. | Obs. | ALS | NC | NHC | Pe |
|---|---|---|---|---|---|---|---|---|
| Ficolin-1 (ng/mL) | EDTA plasma | Log | None | 249 | 242 (219–268) | 246 (217–279) | 253 (229–280) | 0.83 |
| Ficolin-2 (µg/mL) | EDTA plasma | Log | None | 249 | 4.21 (3.77–4.70) | 4.03 (3.51–4.63) | 5.12 (4.58–5.72) | 0.01 |
| Ficolin-3 (µg/mL) | EDTA plasma | Log | None | 249 | 22.8 (21.1–24.7) | 22.4 (20.3–24.7) | 23.0 (21.3–24.9) | 0.92 |
| Collectin-11 (ng/mL) | EDTA plasma | Log | 1 | 249 | 209 (196–223) | 192 (177–208) | 176 (165–188) | 3.9 × 10−4 |
| MB (ng/mL) | EDTA plasma | Sqrt | None | 249 | 949 (784–1130) | 886 (689–1108) | 843 (688–1014) | 0.69 |
| MASP-3 (ng/mL) | EDTA plasma | Log | None | 249 | 5227 (4986–5480) | 534 (5039–5674) | 5081 (4846–5327) | 0.39 |
| MAP-1 (ng/mL) | EDTA plasma | Log | None | 249 | 226 (207–247) | 218 (195–244) | 219 (200–240) | 0.85 |
| PTX-(ng/mL) | EDTA plasma | None | None | 248 | 4.80 (3.40–6.30) | 3.10 (1.40–4.80) | 3.22 (1.92–4.52) | 0.18 |
| C (mg/mL) | Serum | None | None | 249 | 0.23 (0.22–0.24) | 0.21 (0.19–0.23) | 0.19 (0.18–0.20) | 2.1 × 10−4 |
| C4 (AU) | EDTA plasma | Log | None | 249 | 1.3 (1.24–1.52) | 1.3 (1.20–1.55) | 1.5 (1.36–1.66) | 0.36 |
| C3 (mg/mL) | Serum | Log | None | 248 | 1.0 (0.99–1.07) | 1.0 (0.99–1.09) | 1.0 (0.97–1.05) | 0.62 |
| C3bc (AU) | EDTA plasma | Log | None | 249 | 2.97 (2.67–3.30) | 4.59 (4.02–5.25) | 3.30 (2.97–3.67) | 5.1 × 10−7 |
| C3bc/C3 | Index | Log | None | 248 | 2.89 (2.61–3.20) | 4.41 (3.88–5.01) | 3.26 (2.94–3.61) | 5.1 × 10−7 |
| sC5b-(AU) | EDTA plasma | Log | 1 | 249 | 0.66 (0.61–0.71) | 0.58 (0.53–0.64) | 0.58 (0.54–0.63) | 0.017 |
| Ficolin-3 (µg/mL) | CSF | Log | None | 239a | 0.005 (0.0052–0.0067) | 0.004 (0.0038–0.0052) | 0.0049 (0.0043–0.0056) | 0.013 |
| C3bc (AU) | CSF | Log | 2 | 233a, b | 33.4 (27.8–40.2) | 53.6 (42.5–67.6) | 43.3 (36.0–52.1) | 0.0013 |
| C4c (AU) | CSF | Sqrt | None | 235a, c | 3.78 (3.28–4.32) | 3.08 (2.52–3.70) | 3.11 (2.66–3.60) | 0.36 |
| sC5b-9 (AU) | CSF | Data | None | 236a, d | 0.0014 (0.000–0.0030) | 0.001 (0.000–0.0030) | 0.000 (0.000–0.0020 | 0.56 |
Notes: Values are mean (95% confidence interval). aEighty-two ALS patients donated CSF. bSix samples failed during the analysis process and therefore no results are available for six observations in this assay. cFour samples failed during the analysis process and therefore no results were available for four observations in this assay. dThree samples failed during the analysis process and therefore no results were available for four observations in this assay. eAll P values are Bonferroni-corrected (p-value <0.017 to discard the null hypothesis).
Abbreviations: AU, arbitrary unit; NCs, neurologically healthy controls; NHCs, neurologically healthy controls; Log, logarithmically transformed; Sqrt, square root transformed; Transf, transformations; Outl, outliers; Obs, observations; CSF, cerebrospinal fluid.