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. Author manuscript; available in PMC: 2021 May 30.
Published in final edited form as: Nat Mater. 2020 Nov 30;20(4):548–559. doi: 10.1038/s41563-020-00849-5

Extended Data Figure 9: PLOD2 correlations with distant metastasis free survival and probability of relapse free survival.

Extended Data Figure 9:

(a) Log2 scaled data graphs showing relative PLOD2 gene expression levels in patients stratified by estrogen and epidermal growth factor receptor two (ER/HER2) status (ER+ HER2− n=314; ER− or + /HER2+ n=73; ER−/HER2− n=133). Whiskers denote minima and maxima, box denotes 25th and 75th percentiles and median. Statistical analysis was performed to compare PLOD2 expression levels among subtypes using Kruskal-Wallis one-way ANOVA (****p < 0.0001) and two-tailed Mann-Whitney U test without adjustment for multiple comparisons for individual comparisons (****p < 0.0001). (b) Line graphs showing distant metastasis-free survival (DMFS) for patients with estrogen receptor positive and epidermal growth factor receptor two negative breast tumors (ER+/− HER2−; low n=157 & high=157). (c) Line graph of DMFS for patients with estrogen receptor negative or negative and epidermal growth factor receptor two positive breast tumors (ER− or +/HER2+; low n=36 & high=37). (d) Line graph of DMFS for patients with estrogen receptor negative epidermal growth factor receptor negative breast tumors (ER− HER2−; low n=66 & high=67). Statistical analyses were performed to compare PLOD2 and DMFS for each subtype or Log-rank (Mantel-Cox) test (b) p = 0.8051 (c) *p = 0.0144 (d) p = **0.0050. (e-g) Kaplan-Meier curves indicating the probability of relapse-free survival assessed in ER+/PR+ (e), HER2+ (f), and triple negative (g) breast cancer patients up to 16 years after diagnosis. A correlation between PLOD2 (gene encoding LH2) expression and RFS has been determined using an online tool (http://kmplot.com/analysis/) as described in the methods. Top and bottom panels represent two distinct Affymetrix PLOD2 probes (202619 and 202620) from the same database. For Kaplan-Meier curves, statistical analyses were performed using LogRank test.