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. 2021 Mar 15;9:613083. doi: 10.3389/fchem.2021.613083

FIGURE 2.

FIGURE 2

SERS spectra of CD-H2O2-TMB-Apt-AgNP analysis system. A/B compared the catalytic effects between CDFe and CDFeN in the urea analysis system. (A): CDFe-H2O2-TMB-Tris-HCl-Apt-Urea-AgNPs system, a: 0.04 μg/ml CDFe+5.0 μmol/L H2O2+ 0.03 mmol/L TMB+0.51 mmol/L PH = 4.07 Tris-HCl+10 nmol/L Apt+ Urea+0.08 mmol/L AgNPs; b: a+3.33 nmol/L Urea; c: a+6.66 nmol/L Urea; d: a+9.99 nmol/L Urea; e: a+13.32 nmol/L Urea; f: a+16.65 nmol/L Urea. (B): CDFeN-H2O2-TMB-Tris-HCl-Apt-Urea-AgNPs system, a: 0.04 μg/ml CDFeN+ 5.0 μmol/L H2O2+ 0.03 mmol/L TMB+ 0.51 mmol/L PH = 4.07 Tris-HCl+10 nmol/L Apt+ Urea+0.08 mmol/L AgNPs; b: a+3.33 nmol/L Urea; c: a+6.66 nmol/L Urea; d: a+9.99 nmol/L Urea; e: a+13.32 nmol/L Urea; f: a+16.65 nmol/L Urea.