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. 2021 Mar 15;9:613083. doi: 10.3389/fchem.2021.613083

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Copyright © 2021 Li, Li, Liang, Wen and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RRS spectra of CD-Tris-HCl-Apt analysis system. A/B compared the RRS analysis system of urea using CD_Fe and CD_FeN, respectively. (A): CD_Fe-H₂O₂-TMB-Tris-HCl-Apt-Urea-AgNPs system, a: 0.04 μg/ml CD_Fe + 6.7 μmol/L H₂O₂+ 0.03 mmol/L TMB+ 0.84 mmol/L PH = 4.4 Tris-HCl+16.65 nmol/L Apt+0.08 mmol/L AgNPs; b: a+2.5 nmol/L Urea; c: a+5 nmol/L Urea; d: a+7.5 nmol/L Urea; e: a+8.5 nmol/L Urea; f: a+12.5 nmol/L Urea. (B): CD_FeN-H₂O₂-TMB-Tris-HCl-Apt-Urea-AgNPs system, a:0.04 μg/ml CD_FeN + 6.7 μmol/L H₂O₂+ 0.03 mmol/L TMB+ 0.84 mmol/L PH = 4.4 Tris-HCl+16.65 nmol/L Apt+0.08 mmol/L AgNPs; b: a+2.5 nmol/L Urea; c: a+5 nmol/L Urea; d: a+6 nmol/L Urea; e: a+7.5 nmol/L Urea; f: a+10 nmol/L Urea; g: a+12.5 nmol/L Urea.