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. 2021 Mar 15;15:630882. doi: 10.3389/fncel.2021.630882

FIGURE 3.

FIGURE 3

CSF-cNs formed neurospheres and expressed neural stem cell markers. (A,B) The growth of neurospheres in different stages. (A) 0–7 days in the first passage. (B) Passage 1–3. (C–E) Immunofluorescence analysis showing that neurospheres expressed neural stem cell markers for nestin (C), Sox2 (D), and GFAP (E).