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Neurospheres formed by CSF-cNs were tri-potent. (A) Differentiation of neurospheres after adherent culture on days 3 and 7. (B–E) Immunofluorescence analysis showing that neurospheres differentiated into neurons that were positive for Tuj1 (B) and NeuN (C), astrocytes that were positive for GFAP (D), and oligodendrocytes that were positive for O4 (E), respectively. Scale bar represents 50 μm in (A) and 100 μm in (B–E).
