Table 1.
Sporozoite development and infectivity upon ookinete injection in the haemocoel.
| Salivary gland sporozoites | Infectivity to mice | ||
|---|---|---|---|
| Parasite | Mean | SEM | |
| c507 | 2,907 (3036;1888;4850;2842;2175;2650) | 389 | 12/12 (2/2;2/2;ND;3/3;3/3;2/2) |
| Δc01 | 973 (582;683;492;2653;824;603) |
310 | 3/12 (0/2;0/2;ND;2/3;1/3;0/2) |
| Δc57 | 3,460 (2328;6955;3864;2105;3050;2458) |
680 | 12/12 (2/2;2/2;ND;3/3;3/3;2/2) |
| Δc22 | 501 (995;216;560;586;396;252) |
107 | 0/12 (0/2;0/2;ND;0/3;0/3;0/2) |
Mean salivary gland sporozoite numbers 21 days post A. coluzzii haemocoel inoculation with ookinetes, obtained from six biological replicates. Data from independent biological replicates are presented in brackets and are separated with semicolons. SEM represents the standard error of the mean. Infectivity of sporozoites was assessed by infected mosquito bite back experiments of C57/BL6 mice at day 21 post haemocoel inoculation. Parasitemia was monitored for 14 days post mosquito bite and the ratio of infected to all mice used is shown. ND, not determined.