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. 2021 Mar 18;6(10):3343–3357. doi: 10.1016/j.bioactmat.2021.02.039

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — SIRT3 treatment mitigated Ti particle-induced suppression of osteogenesis and mineralization in rMSCs. (A) Representative images of immunofluorescence staining; red (OCN), green (Phalloidin), and blue (nuclei). (B) Representative images of ALP staining at 7 days. (C) Representative images of ARS staining at 21 days. (D) Semiquantitative evaluation of fluorescence intensity. (E) Quantitative evaluation of ALP activity. (F) Semiquantitative evaluation of the ARS recovery ratio. (n = 6 per group. The cell seeding density was 5 × 10³ cells per well in 24-well plates. Values are shown as the means ± s.d. **p < 0.01 and ***p < 0.005).