Fig. 6.
SIRT3 treatment suppressed Ti particle-stimulated activation of the pyroptosis-related NLRP3 inflammasome. (A) Representative images of IHC staining, identified with black arrows showing NLRP3-positive cells. (B) Western blot of pyroptosis-related proteins. (C) Semiquantitative evaluation of NLRP3-positive cells (n = 6 per group). (D-J) Protein levels of NLRP3, caspase-1, GSDMD, IL-1β, and IL-18, n = 3 per group. (K-L) Secretion of IL-1β and IL-18 in the culture medium, n = 6 per group. (The cell seeding density was 1 × 105 cells per well in 6-well plates. Values are shown as the means ± s.d. *p < 0.05, **p < 0.01 and ***p < 0.005).