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. 2021 Mar 16;21(5):485. doi: 10.3892/etm.2021.9916

Figure 2.

Figure 2

Relative ACE2 expression in cells expressing SARS-CoV-2 ORF3a. (A) A549, DOK and HaCaT cells were transduced for 24 h with lentiviral particles carrying ORF3a from SARS-CoV-2 and were subsequently treated with Vita Deyun solution. The expression of ACE2 was determined by reverse transcription-quantitative PCR, with RPL32 and RPS18 serving as reference genes and the value of untreated cells as the control (2-ΔΔCp algorithm). The values are presented as the mean ± SD from at least three different experiments. *P<0.05. (B) Data from project GSE152586 of the Gene Expression Omnibus were used to determine variations in ACE2 expression following 48 h of SARS-CoV-2 infection in alveolar spheroids. The values are expressed in fragments per kilobase of exon model per million reads mapped (FPKM), which is a normalized estimation of gene expression based on RNA-seq data. ACE2, angiotensin-converting enzyme 2; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; ORF3a, open reading frame 3a; RP, ribosomal protein.