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. 2021 Mar 16;21(5):483. doi: 10.3892/etm.2021.9914

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Flow cytometric analysis of Treg cells. CD25-FITC, FoxP3-PE and isotype control antibodies were used to determine the proportion of Treg lymphocytes. (A) Percentage of Treg cells in WKY-C, WKY-M, and WKY-T groups. (B) Percentage of Treg cells in week 16 was analyzed using the one-way ANOVA with a Tukey's post hoc test. Data are presented as the mean ± standard error of the mean. n=3/group. ^***P<0.001 and ^****P<0.0001 as indicated. C, control; M, methionine; T, treatment; WKY, Wistar-Kyoto; Treg cells, regulatory T cells; FoxP3, forkhead box P3.