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. 2021 Mar 17;21(5):497. doi: 10.3892/etm.2021.9928

Figure 2.

Figure 2

SNHG14 knockdown alleviates inflammation and apoptosis of PC-12 cells induced by LPS. (A) SNHG14 expression in PC-12 cells after LPS induction was detected by RT-qPCR. ***P<0.001 vs. Control group. (B) The transfection efficiency of shRNA-SNHG14-1/2 was verified by RT-qPCR. ***P<0.001 vs. Control group. ###P<0.001 vs. shRNA-NC group. ∆∆P<0.01 vs. shRNA-SNHG14-1 group. (C) LPS-induced PC12 cell viability after transfection was detected by Cell Counting Kit-8 assay. *P<0.05 and ***P<0.001 vs. Control group. ##P<0.01 vs. 5 µg/ml LPS group. ∆∆P<0.01 vs. 5 µg/ml LPS + shRNA-NC group. (D) LPS-induced PC12 cell apoptosis after transfection was detected by TUNEL assay. Magnification, x200. ***P<0.001 vs. Control group. ###P<0.001 vs. 5 µg/ml LPS group. ∆∆∆P<0.001 vs. 5 µg/ml LPS + shRNA-NC group. (E) The expression of apoptosis-related proteins was analyzed by western blot analysis and (F) quantified. *P<0.05 and ***P<0.001 vs. Control group. #P<0.05, ##P<0.01 and ###P<0.001 vs. 5 µg/ml LPS group. ∆P<0.05, ∆∆P<0.01 and ∆∆∆P<0.001 vs. 5 µg/ml LPS + shRNA-NC group. (G) The levels of TNF-α, IL-1β and IL-6 in the supernatant of LPS-induced PC12 cells after transfection were detected by ELISA. **P<0.01 and ***P<0.001 vs. Control group. ###P<0.001 vs. 5 µg/ml LPS group. ∆∆∆P<0.001 vs. 5 µg/ml LPS + shRNA-NC group. SNHG14, small nucleolar host gene 14; LPS, lipopolysaccharide; shRNA, short hairpin RNA; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR.