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. 2021 Mar 17;21(5):497. doi: 10.3892/etm.2021.9928

Figure 3.

Figure 3

SNHG14 negatively regulates miR-181b-5p in PC-12 cells. (A) miR-181b-5p expression in PC12 cells after LPS induction was detected by RT-qPCR. ***P<0.001 vs. Control group. (B) miR-181b-5p expression in PC12 cells transfected with shRNA-SNHG14-1 was detected by RT-qPCR. ***P<0.001 vs. Control group. ###P<0.001 vs. shRNA-NC group. (C) The transfection efficiency of miR-181b-5p mimic was verified by RT-qPCR. ***P<0.001 vs. Control group. ###P<0.001 vs. mimic-NC group. (D) SNHG14 expression in PC-12 cells transfected with miR-181b-5p mimic was detected by RT-qPCR. ***P<0.001 vs. Control group. ###P<0.001 vs. mimic-NC group. (E) Binding sites between SNHG14 and miR-181b-5p. (F) Luciferase reporter assay verified the direct binding relationship between SNHG14 and miR-181b-5p. **P<0.01 vs. mimic-NC group. SNHG14, small nucleolar host gene 14; LPS, lipopolysaccharide; shRNA, short hairpin RNA; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR; miR, microRNA; WT, wild-type; MUT, mutant; luc, luciferase; R-Luc, Renilla luciferase; lncRNA, long non-coding RNA.