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. 2021 Mar 15;12:651109. doi: 10.3389/fimmu.2021.651109

Figure 2.

Figure 2

Macrophages and HUVEC internalize MP in a dose-, time-, and temperature-dependent manner. MSC were labeled with PKH-26 before the generation of MP (PKH-MP). PKH-MP were added to macrophages or HUVEC (ratios 1:10,000, 1:50,000, 1:100,000), incubated for 1, 6, and 24 h at 4 or 37°C, and analyzed by flow cytometer or confocal microscopy. (A) Percentage of macrophages and HUVEC positive for PKH-MP in different doses of MP over time, and (B) at 4 and 37°C over time. (C) Representative confocal microscopy analysis of MP uptake by macrophages and HUVEC (ratio 1:50,000) at time point 24 h. Staining for CD81 cell membrane (green), Hoechst 33,342 nucleus (blue), and PKH26-MP (red) demonstrated that MP are internalized by macrophages and HUVEC. Data are presented as mean ± SD from 3 to 4 experiments. ***P < 0.001 vs. ratio 1:10,000 and ###P < 0.001 vs. ratio 1:50,000 in (A); **P < 0.001 and **P < 0.001 vs. 4°C in (B). Scale bars: 20 μm (macrophages) and 50 μm (HUVEC).