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. 2021 Mar 15;12:651109. doi: 10.3389/fimmu.2021.651109

Figure 5.

Figure 5

MP uptake under pro- and anti-inflammatory conditions modulates macrophages function. Macrophages were primed with 100 ng/ml LPS and 20 ng/ml IFNγ (M1 macrophages) or with 20 ng/ml IL-4 and 20 ng/ml IL-13 (M2 macrophages). (A,B) PKH-MP were added to macrophages (ratio 1:50,000) and incubated for 24 h for uptake analyze by flow cytometer and confocal microscopy. (A) Percentage of macrophages positive for PKH-MP in different culture conditions. (B) Representative confocal microscopy analysis of MP uptake by macrophages under stimulation of IFNγ + LPS or IL-4 + IL-13. (C,D) Macrophages were separated from MP and assessed by real-time RT-PCR after 48 h. mRNA expression of macrophages treated with MP in the presence of (C) LPS + IFNγ (CXCL10, CCR7, IL-1, IL-10, TNFα, and TGFβ) and (D) IL-4 + IL-13 (CCL22 and CD209). Data are presented as mean ± SD from 6 experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. medium control or vs. macrophages without MP and #P < 0.05 vs. macrophages stimulated with IL-4 + Il-13. Scale bar: 20 μm.