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. 2021 Mar 15;12:608625. doi: 10.3389/fimmu.2021.608625

Figure 2.

Figure 2

Inhibition of PI3K∂ by idelalisib leads to decreased T-cell-mediated cytotoxicity against target tumor cells and reduced secretion of IL-10, TNF, and IFNγ. (A) Cytolytic capacity of CD3+ T cells in coculture with target HL-60 cell line and various concentrations of idelalisib after 72 h. (B) Bar charts and representative overlaid flow cytometric histograms depicting expression levels of perforin and granzyme B in CD8 T cells after bead mediated activation for 72 h in the presence of different concentrations of idelalisib or DMSO. (C) Bar charts representing the levels of IL-2, IL-10, TNF, and IFNγ in the supernatant of the coculture after 72 h. Error bars represent mean ± SEM; *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, n = 12 HDs.