Figure 5.

CD45RO(A_2AR)-NPs rescue the chemotactic ability of HNSCC CD8⁺ memory T cells in the presence of adenosine

(A and B) Representative two point trajectories of T cells migrating along the CXCL10 gradient (green) or CXCL10 + adenosine (ADO) gradient (blue) in HNSCC CD8⁺ memory T cells treated with (A) CD45RO(scr)-NPs or (B) CD45RO(A_2AR)-NPs. Trajectories artificially set to start at the origin with the red triangle representing the Y-COM. (C) Y-COM of HNSCC CD8⁺ memory T cells treated with (left) CD45RO(scr)-NPs (n = 6) or (right) CD45RO(A_2AR)-NPs (n = 6) in the presence of CXCL10 or CXCL10 + adenosine. Significance was determined using (left) a Wilcoxon signed rank test or (right) paired Student’s t test. (D) Percent inhibition of Y-COM of HNSCC CD8⁺ memory T cells in the presence of CXCL10 + adenosine compared to CXCL10 alone when treated with CD45RO(scr)-NPs (n = 6) compared to CD45RO(A_2AR)-NPs (n = 6). Data are represented single dot plots (colored dots; wherein each dot represents a single donor) and as mean ± standard deviation (shown in black). Significance was determined using Student’s t test.