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. 2021 Mar 15;12:651217. doi: 10.3389/fmicb.2021.651217

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Copyright © 2021 Shan, Jia, Teulières, Patel and Clokie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A phylogenetic tree constructed based on the terL nucleotide sequence. The LD and RF Borrelia species and B. miyamotoi are separated into three well-supported clades. The Molecular Evolutionary Genetics Analysis (MEGA) package version 7 was used, and the tree was constructed via the neighbor-joining method. Scale bar represents the units of the number of base substitutions per site. Support for the clades was estimated via bootstrap analysis in MEGA with 3,000 replicates, values are indicated at the nodes (only values greater than 75 are displayed). The terL sequence from phage lambda was used as an outgroup to root the tree.