FIGURE 4.

Performance of the Ter-qPCR estimated by examining human blood spiked with tenfold serial dilutions of Borrelia cells (10⁵ to 0.1). (A): bar graph illustrating the number of spike-in Borrelia cells and the resulting copy numbers determined by the Ter-qPCR; (B): Linear regression analysis between the known amount of spiked Borrelia cells and the resulting Cq values revealed a strong linear association. The slope, coefficient of correlation (R²) and efficiency of the reaction (E) are shown. Each dot represents the average value from triplicate repeats along with SD values obtained from two independent experiments.