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. 2021 Mar 6;24(4):102275. doi: 10.1016/j.isci.2021.102275

Figure 5.

Figure 5

Patency for lipid uptake by the oocyte at St 10a

(A–C””) Lipids are present in the gaps in patent FE. (A–B””) Nile red staining shows lipids in the TC gaps and spanning the FE. Basal and cross-sectional views (A′–A”” and B′–B””, respectively). CD8-GFP marks the membrane. (C–C””) BODIPY493/503 (green) confirms presence of lipids in the gaps. Membrane is marked by Hts (red).

BODIPY staining of control WT St 10a egg chamber (D–D″) and UAS-Dpp expressing FE lacking patency showing reduced levels of oocyte lipids at St 10a (E–E″). Note FE lipid globules are present in both.

(F) Quantification of the intensity of BODIPY signal per unit area in oocytes surrounded by UAS-Dpp expressing FE and WT FE at St 10a (N = 10).

(F′) Phenotype variation observed indicating FE lacking TC gaps and FE with WT phenotype (TC gaps present).

(G and G′) Later stage egg chambers with WT FE and UAS-Dpp expressing FE, resulting in abnormal egg chambers.

(G″) Quantification of cross-sectional area of the oocyte as a measure of oocyte growth in WT egg chambers and patency-lacking egg chambers with UAS-Dpp expressing FE (N = 10; data are represented as mean ± SEM). DAPI marks the nuclei. Scale bars: (A-C) and (E-F″), 20 μm; (A′-C""), 2 μm; (G-G′), 50 μm.