Table 1.
Composition of the experimental diets (g/kg, as fed-basis).
| Item | 1–21 d |
22–42 d |
||
|---|---|---|---|---|
| PC1 | NC1 | PC | NC | |
| Ingredients | ||||
| Corn | 544.13 | 578.75 | 662.48 | 680.55 |
| Soybean meal (46% CP) | 380.60 | 374.70 | 269.10 | 266.20 |
| Soybean oil | 32.00 | 3.11 | 28.30 | 13.10 |
| Limestone | 10.40 | 10.46 | 11.00 | 10.98 |
| Monocalcium phosphate | 15.61 | 15.57 | 9.51 | 9.52 |
| NaCl | 4.82 | 4.81 | 4.49 | 4.48 |
| Lysine sulfate (50.7% Lys) | 2.48 | 2.66 | 4.03 | 4.10 |
| DL-Methionine (99% Met) | 2.93 | 2.90 | 2.81 | 2.79 |
| L-Threonine (99% Thr) | 0.41 | 0.41 | 0.97 | 0.97 |
| L-Valine (93.5% Val) | 0.02 | 0.03 | 0.71 | 0.71 |
| Choline chloride (60% Chol) | 0.60 | 0.60 | 0.60 | 0.60 |
| Vitamin-mineral premix2 | 2.50 | 2.50 | 2.50 | 2.50 |
| Mycotoxin absorbent3 | 2.50 | 2.50 | 2.50 | 2.50 |
| Inert4 | 1.00 | 1.00 | 1.00 | 1.00 |
| Calculated composition | ||||
| Metabolizable energy (kcal/kg) | 2,983 | 2,833 | 3,125 | 2,975 |
| Crude protein | 220.0 | 220.0 | 187.5 | 187.5 |
| Calcium | 8.53 | 8.53 | 6.85 | 6.85 |
| Available phosphorus | 4.18 | 4.18 | 3.20 | 3.20 |
| Dig Lysine | 12.19 | 12.19 | 10.44 | 10.44 |
| Dig Methionine + Cystine | 8.79 | 8.79 | 7.62 | 7.62 |
| Dig Threonine | 7.93 | 7.93 | 6.78 | 6.78 |
| Dig Tryptophan | 2.46 | 2.46 | 1.88 | 1.88 |
| Dig Valine | 9.39 | 9.39 | 8.14 | 8.14 |
| Chloride | 3.40 | 3.40 | 3.21 | 3.21 |
| Sodium | 2.10 | 2.10 | 1.97 | 1.97 |
| Potassium | 8.54 | 8.54 | 7.60 | 7.60 |
PC, positive control (meeting the nutritional requirements of broilers without the enzyme inclusion); NC, negative control (reduction of 100 kcal/kg).
Vitamins and minerals provided per kg of feed: vitamin A, 20,000 UI; vitamin D3 7,500 UI; vitamin E 50 mg; vitamin K3 5.25 mg; vitamin B1 4.5 mg; vitamin B2 13.75 mg; vitamin B6 6.25 mg; vitamin B12 37.5 mg; pantothenic acid 27.5 mg; niacin 87.5 mg; folic acid 2.25 mg; biotin, 0.16 mg; selenium, 0.75 mg; copper, 20 mg; iron, 112.5 mg; manganese, 200 mg; cobalt, 5 mg; iodine, 3 mg; zinc, 275 mg.
Bentonite and activated carbon.
The different enzyme combinations were added in replacement for inert (Kaolin) amylase (RONOZYME HiStarch, 80 KNU/kg); amylase + xylanase (RONOZYME WX, 100 FXU/kg); and amylase + xylanase + protease (RONOZYME ProAct, 15,000 PROT/kg).