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c(M) distribution of unfractionated VWF/fVIII complexes. The A280 scans (Fig. 1A) and interference scans (Fig. 1B) of heparin-Sepharose purified VWF/FVIII were fit the continuous c(M) distribution model in SEDFIT using a fixed frictional ratio of 3.0 as described in Experimental procedures. Red, absorbance; blue, interference. c(M) values are normalized to the maximum value.
