Table 1.
SV and DLS analysis of SEC-fractionated VWF/fVIII complexes
| Sample | (sw)20,w (S) |
(Dz)20,w × 107 (cm2 s−1) | PDI | Rh (nm) | MW (MDa) |
f/fo |
|||
|---|---|---|---|---|---|---|---|---|---|
| A280 | IF | A280 | IF | A280 | IF | ||||
| A | 27.74 (27.52, 27.97) | 26.81 (27.74, 26.88) | 0.620 (0.609, 0.631) | 0.26 | 32.3 | 4.10 | 3.92 | 3.37 | 3.42 |
| B | 25.80 (25.63, 26.96) | 25.65 (25.59, 25.75) | 0.650 (0.647, 0.653) | 0.24 | 30.8 | 3.66 | 3.59 | 3.33 | 3.36 |
| C | 23.27 (23.11, 23.44) | 23.24 (23.19, 23.32) | 0.712 (0.706, 0.718) | 0.24 | 28.2 | 3.04 | 2.99 | 3.22 | 3.26 |
| D | 20.45 (20.37, 20.62) | 20.70 (20.65, 20.75) | 0.792 (0.787, 0.797) | 0.16 | 25.3 | 2.43 | 2.42 | 3.10 | 3.15 |
| E | 16.84 (16.73, 16.99) | 16.80 (16.70, 16.95) | 0.946 (0.943, 0.949) | 0.24 | 21.2 | 1.71 | 1.68 | 2.90 | 2.98 |
A280, absorbance at 280 nm; IF, interference.
Numbers in parentheses represent 95% confidence intervals.
(sw)20,w: Weight-average sedimentation coefficient adjusted the standard condition of 20 °C in water.
(Dz)20,w: z-average diffusion coefficient adjusted to the standard condition of 20 °C in water.
PDI: polydispersity index (Equation 6).
Rh: Hydrodynamic radius (Equation 7).