Fig. 2.

(A) GFP expression in the hypothalamic ARC after stereotaxic injection of shSIRT3 lentivirus (B) Protein levels of SIRT3 in the ARC of male rats stereotaxically injected with scrambled or shSIRT3 lentiviruses. (C) Effect on body weight (D) and food intake and (E) Fat mass. Fasting levels of glucose (F) and insulin (G) glucose tolerance test (H) and area under the curve (I) insulin tolerance test (J) and area under the curve (K) in male rats fed chow diet that received shSIRT3 or GFP scrambled lentiviruses in the ARC. β-actin was used to normalize protein levels. Dividing lines indicate spliced bands from the same gel. Values are mean ± SEM of 7–18 animals per group. *P ≤ 0.05, **P ≤ 0.01 vs controls (Paired t-test and two way ANOVA).