Fig. 3.

(A) Protein levels of NPY, AgRP and POMC in the hypothalamic ARC of male rats stereotaxically injected with scrambled or shSIRT3 lentiviruses in the ARC (B) Representative infrared thermal images (C) BAT temperature (D) representative pictures of BAT histology and (E) BAT protein levels. (F) Representative pictures of WAT histology and (G) WAT protein levels of pHSL, HSL, ACC, FAS and LPL (H) WAT protein levels of UCP-1, UCP-3, PRDM16 and CIDEA in male rats fed chow diet injected with shSIRT3 or GFP scrambled lentiviruses into the ARC. α-tubulin, GAPDH or β-actin were used as loading control. Dividing lines indicate spliced bands from the same gel. Values are mean ± SEM of 7–18 animals per group. *P ≤ 0.05, **P ≤ 0.01 vs controls (Paired t-test).