Fig. 5.

(A) Immunofluorescence picture showing that GFP infection was restricted to POMC neurons. (B) Effect on body weight (C) food intake (D) energy expenditure (E) locomotor activity (F) respiratory quotient (G) Basal blood glucose levels (H) glucose tolerance test and (I) area under the curve in male mice fed chow diet that received AAV8-hSyn-shSIRT3-DIO-GFP or AAV8-hSyn-DIO-GFP scrambled viruses in the POMC neurons. Values are mean ± SEM of 6–7 animals per group. *P ≤ 0.05, **P ≤ 0.01 vs controls (Paired t-test and two way ANOVA).