Figure 3.

Effect of anionic and non-bilayer lipids on Lyp1 activity in proteoliposomes. (a) Turnover number of Lyp1 as a function of POPS. (b) Turnover number of Lyp1 as a function of POPE (mol%). (c) Turnover number of Lyp1 in vesicles with POPE versus POPA. (d) Turnover number of Lyp1 as a function of DOPE, which was increased at the expense of POPE. The triangles at the top of each graph depict the gradual replacement of one lipid for another. Number of replicate experiments (n) = 3; the variation between replicates is ±20%, and therefore, the error bars are the standard error of the fit of n = 1. Sigmoidal curves were fitted using the equation: $y=start+\left(end-start\right)\frac{x^n}{k^n+x^n}$