KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD9 antibody | Abcam | Cat# ab92726; RRID: AB_10561589 |
| c-Myc antibody | Cell Signaling Technology | Cat# 5605; RRID: AB_1903938 |
| FAK antibody | Cell Signaling Technology | Cat# 3285; RRID: AB_2269034 |
| Fibronectin antibody | Sigma-Aldrich | Cat# F3648; RRID: AB_476976 |
| FlotiNin-2 antibody | Cell Signaling Technology | Cat# 3436; RRID: AB_2106572 |
| Klf4 antibody | Cell Signaling Technology | Cat# 4038; RRID: AB_2265207 |
| Nanog antibody | Abcam | Cat# ab80892; RRID: AB_2150114 |
| Oct3/4 antibody | Santa Cruz Biotechnology | Cat# sc-5279; RRID: AB_628051 |
| Phospho-FAK (Tyr397) antibody | Cell Signaling Technology | Cat# 3283; RRID: AB_2173659 |
| Sox2 antibody | Cell Signaling Technology | Cat# 4900; RRID: AB_10560516 |
| Tri-Methyl-Histone H3 (Lys27) antibody | Cell Signaling Technology | Cat# 9733; RRID: AB_2616029 |
| Vinculin antibody | Cell Signaling Technology | Cat# 13901; RRID: AB_2728768 |
| Rabbit IgG-HRP conjugate antibody | Cell Signaling Technology | Cat# 7074; RRID: AB_2099233 |
| Mouse IgG-HRP conjugate antibody | Cell Signaling Technology | Cat# 7076; RRID: AB_330924 |
| Mouse IgG-Alexa 488 conjugate antibody | Thermo Fisher Scientific | Cat# A-11029; RRID: AB_2534088 |
| Rabbit IgG-Alexa 568 conjugate antibody | Thermo Fisher Scientific | Cat# A-11036; RRID: AB_10563566 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Aprotinin | Sigma-Aldrich | Cat# 10236624001 |
| B-27 supplement | Thermo Fisher Scientific | Cat# 17504044 |
| Bio-Rad protein assay | Bio-Rad | Cat# 5000006 |
| CHIR99021 | Selleckchem | Cat# S1263 |
| DAPI | Sigma-Aldrich | Cat# D9542 |
| Dimethylsulfoxide (DMSO) | Sigma-Aldrich | Cat# D8418 |
| Dithiothreitol (DTT) | Sigma-Aldrich | Cat# 10197777001 |
| DMEM | Gibco | Cat# 11965-092 |
| DMEM/F12 | Gibco | Cat# 12634-010 |
| Western Lightning Plus-ECL | Perkin-Elmer | Part# NEL105001EA |
| FAK inhibitor III | Sigma-Aldrich | Cat# 5040450001 |
| Y15 | Sigma-Aldrich | Cat# SML0837 |
| Fetal bovine serum | Gibco | Cat# 10437028 |
| Fibronectin (Bovine plasma) | Millipore | Cat# 341631 |
| FM1-43FX | Thermo Fisher Scientific | Cat# F35355 |
| Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) | Sigma-Aldrich | Cat# SCP0157 |
| KSOM medium | Sigma-Aldrich | Cat# MR-101-D |
| L-Ascorbic acid | Millipore | Cat# 1831 |
| Leukemia inhibitory factor (mouse) | Millipore | Cat# ESG1107 |
| Leupeptin | Sigma-Aldrich | Cat# L9783 |
| L-Glutamine | Sigma-Aldrich | Cat# G8540 |
| M2 medium | Sigma-Aldrich | Cat# MR-015-D |
| Monothioglycerol | Sigma-Aldrich | Cat# M6145 |
| N-2 supplement | Thermo Fisher Scientific | Cat# 17502048 |
| Neurobasal medium | Gibco | Cat# 21103049 |
| Phosphate buffered saline tablets | VWR | Cat# VWRVE404 |
| PD03259010 | Selleckchem | Cat# S1036 |
| Pen-Strep | Gibco | Cat# 15140122 |
| RNase A | Thermo Fisher Scientific | Cat# EN0531 |
| StemPro accutase | Thermo Fisher Scientific | Cat# A1110501 |
| Trypan Blue solution 0.4 % | Gibco | Cat# 15250061 |
| Trypsin-EDTA (0.05 %) | Gibco | Cat# 25300054 |
| Critical Commercial Assays | ||
| VECTOR Red Alkaline phosphatase substrate kit | VECTOR laboratories | Cat# SK-5100 |
| RNeasy mini kit | QIAGEN | Cat# 74104 |
| Superscript III Reverse transcriptase | Invitrogen | Cat# 18080044 |
| Deposited Data | ||
| Unprocessed blots | This paper | https://doi.org/10.17632/mt4gm74tbw.1 |
| Experimental Models: Cell Lines | ||
| Mouse: E14tg2a.4 | ATCC | Cat# CRL-1821 |
| Mouse: Mouse Embryonic Fibroblasts | Cornell’s Stem Cell and Transgenic Core Facility | N/A |
| Mouse: NIH3T3 | ATCC | Cat# CRL-1658 |
| Mouse: v6.5 | Provided by Cornell’s Stem Cell and Transgenic Core Facility, Cornell University | N/A |
| Oligonucleotides | ||
| Primer: Oct3/4 Forward: GTTGGAGAAGGTGGAACCAA | This paper | N/A |
| Primer: Oct3/4 Reverse: CCAGTCACACCCAACCTCTT | This paper | N/A |
| Primer: Nanog Forward: CATGTTTAAGGTCGGGCTGT | This paper | N/A |
| Primer: Nanog Reverse: AAAGAGTGCGTGGAGGAAGA | This paper | N/A |
| Primer: Sox2 Forward: AGGGCTGGGAGAAAGAAGAG | This paper | N/A |
| Primer: Sox2 Reverse: TTGCTGATCTCCGAGTTGTG | This paper | N/A |
| Primer: Klf4 Forward: CAGCTTCATCCTCGTCTTCC | This paper | N/A |
| Primer: Klf4 Reverse: CGCCTCTTGCTTAATCTTGG | This paper | N/A |
| Primer: c-Myc Forward: ATGCATTTTGAAGCGGGGTT | This paper | N/A |
| Primer: c-Myc Reverse: CAACGAATCGGTCACATCCC | This paper | N/A |
| Primer: Lin28 Forward: AGGAGAAGCTGAGGTGTCCA | This paper | N/A |
| Primer: Lin28 Reverse: ACAAGTGTTGGGCCTATTGC | This paper | N/A |
| Primer: GAPDH Forward: CTCAGCTCCCCTGTTTCTTG | This paper | N/A |
| Primer: GAPDH Reverse: CCTTCCACAATGCCAAAGTT | This paper | N/A |
| Primer: β-actin Forward: TGTTACCAACTGGGACGACA | This paper | N/A |
| Primer: β-actin Reverse: GACATGCAAGGAGTGCAAGA | This paper | N/A |
| Recombinant DNA | ||
| pLV-neo-CD2-FAK | Shibue and Weinberg, 2009 | Addgene Plasmid #37013 |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej-nih-gov.proxy.library.cornell.edu/ij/ |
| IPLABs | BD Biosciences | N/A |
| Prism | Graphpad | https://www.graphpad.com |
| Other | ||
| 0.22 mm Steriflip filter | Millipore | Cat# SEM1M179M6 |
| Amicon Ultra-15 centrifugal filter units – 100 Kda | Millipore | Cat# UFC910024 |
| NanoDrop spectrophotometer | Thermo Fisher Scientific | Cat# 840-274200 |
| NanoSight NS300 | Malvern | N/A |
| Nunc Lab-Tek 8 well permanox chamber slide | Thermo Fisher Scientific | Cat# 177445 |
| SW 41 Ti rotor | Beckman Coulter | N/A |
| Type 45 Ti rotor | Beckman Coulter | N/A |
| Ultra low attachment plate (6 well) | Corning | Cat# 3471 |