Figure 3. An example one-step RT-PCR gel image for the primer design.

Three sets of primers targeting same MERS-CoV genome were tested. A serial dilution of MERS-CoV RNA up to 10^-6 dilution was used to test the primers. The SSIII one-step PCR system was used for the amplification. PCR products were examined by gel electrophoresis with 1% agarose gel. Primer set 2 had the best amplification efficiency thus it was selected for downstream Primer ID primer design. NC, no-reaction control.