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The IgG WB is the less time-consuming method in comparison to albumin Western Blot and AIM. The time investment for the single steps of the three different methods to evaluate BBB disruption was identified for a group size of eight mice. The first listed time period depicts the hands-on time, during which the scientist is tied to the respective procedure. The second time period comprises the active steps of the procedure as well as the preparation and post processing steps, if needed.
