Table 3.

Reaction mix for adding A-tails to the PCR products.

Reagent	Volume Per Reaction (µL)
PCR product (0.05–0.1 µg/µL)	7.0
dATP/dNTP (10 mM)	1.0
10× PCR buffer	1.0
Taq Polymerase (5 U/ µL)	1.0
Total	10