Table 5.

Reaction mix for colony PCR.

Reagent	Volume Per Reaction (µL)
10× PCR buffer	1
dNTP mix (10 mM)	0.5
Internal forward primer (10 pmol/µL)	0.5
Internal reverse primer (10 pmol/µL)	0.5
Taq polymerase (5 U/µL)	0.2
d H2O	15.3
Bacterial suspension	2
Total	20