Table 10.

Reaction mix for anti-sense probe preparation.

Reagent	Volume Per Reaction (µL)
Purified PCR product (0.05–0.1 µg/µL)	6.5
10× NTP labeling mixture	1
10× Transcription buffer	1
Protector RNAse inhibitor	0.5
Sp6 RNA polymerase or T7 RNA polymerase	1
Total	10