Table II.
Proteolytic activity of HCoV PL1pro mutants
| Region of HCoV PL1pro | Replacements in pp1a/pp1ab (from → to)a | PLpro whose sequence at this position matches the HCoV PL1 mutant | Proteolytic activityb | Reference |
|---|---|---|---|---|
| Zinc finger | C1126D, H | No | This work | |
| V1126/27ins | HCoV PL2 | No | This work | |
| C1128A, D, P | No | This work | ||
| C1154A, H, D | No | This work | ||
| L1155del | MHV PL1/HCoV PL2 | No | This work | |
| C1157A, D, P | No | This work | ||
| H | HCoV PL2/TGV PL2 | No | ||
| C1163A | IBV PL | Yes | This work | |
| D | Yes | |||
| Outside of zinc finger | K1048E | IBV PL | No | This work |
| C1054S, A, G | No | 41 | ||
| G1099P | MHV PL1 | Yes | This work | |
| A | No | |||
| G1102S | Yes | This work | ||
| A | MHV PL2/IBV PL | Yes | ||
| V1175H | MHV PL1 | No | This work | |
| P | No | |||
| N | Yes | |||
| T | TGV PL2 | Yes | ||
| C1203D | MHV PL1 | Yes | This work | |
| A | No | |||
| H1205A, G | No | 41 | ||
| D1218N, Q, E, | Yes | This work | ||
| H, K, A | Yes |
a
V1126/27ins, insertion of Val between aa 1126 and 1127; L1155del, deletion of L1155.
b
Proteolytic activity was monitored using SDS-polyacrylamide gel electrophoresis of the products of reactions catalyzed by anin vitro produced PL1pro or its derivatives in the trans-cleavage and monomolecular assays (41).