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. 2021 Mar 29;19(3):e06498. doi: 10.2903/j.efsa.2021.6498

Table 2.

Overview of parameters in OECD guidelines

OECD no. Species pH Temperature (°C) TOC CaCO3 (mg/L) Hardness
203, 210 Fish, e.g. Oncorhynchus mykiss 6.0–8.5 20–25, resp. 13–17 < 2 mg/L
202, 211 Aquatic invertebrates, e.g. Daphnia magna 6.0–9.0 18–22 < 2 mg/L 140–250
218, 219 Chironomus riparius 6.0–9.0 20 ± 2°C
225 Lumbriculus sp. 6.0–9.0 20 ± 2°C
201 Algae 7.5–8.0 21–24 ± 2°C < 0.6 mg/L
211 Lemna sp. 6.5/7.5 24 ± 2°C
238 Myriophyllum sp. 6.0–9.0 23 ± 2°C
239 Myriophyllum sp. (with sediment) 7.5–8.0 20 ± 2°C