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Spin label structures and the GB1 constructs used in this work. (a) MTSL nitroxide conjugated to a cysteine residue, resulting in the R1 side chain (top) and CuII–NTA coordinated to a double-histidine motif (bottom). (b) Cartoon representation of the I6R1/K28H/Q32H GB1 construct, with the R1 nitroxide and CuII–NTA spin labels shown in stick representation. (c) Cartoon representation of the K28H/Q32H GB1 construct, with the CuII–NTA shown in stick representation.
