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. Author manuscript; available in PMC: 2021 Mar 29.
Published in final edited form as: Kidney Int. 2011 Jan 26;79(8):861–870. doi: 10.1038/ki.2010.527

Figure 2 |. Effect of heat shock protein 70 (Hsp70) deficiency or Hsp70 induction by geranylgeranylacetone (GGA) on postischemic renal function.

Figure 2 |

(a) Serum blood urea nitrogen (BUN) and creatinine at baseline and after 25 min of bilateral renal pedicle occlusion (RPO) in Hsp70+/+, Hsp70+/−, and Hsp70−/− mice; *P<0.05 vs Hsp70+/+; †P<0.05 vs Hsp70+/+ after ischemia, n = 6. (b) Serum BUN and creatinine at baseline and after 40 min of bilateral RPO in Hsp70+/+ mice with and without pretreatment with GGA; ∇ indicates animal death; *P<0.05 GGA vs vehicle alone; n = 6. (c) Serum BUN and creatinine levels at baseline and after 25 min of bilateral RPO in Hsp70+/− mice with and without GGA pretreatment; *P<0.05 for GGA vs vehicle alone; n = 6. (d) Serum BUN and creatinine levels at baseline and after 21 min of bilateral RPO in Hsp70−/− mice with and without pretreatment with GGA; NS P>0.05 for GGA vs vehicle; n = 6. (e) Immunoblot analysis of Hsp70 content in renal cortical homogenates harvested from Hsp70+/+, Hsp70+/−, and Hsp70−/− mice after treatment with GGA (400 mg/kg) or vehicle treatment; GGA increased Hsp70 expression only in Hsp70+/+ and Hsp70+/− mice; densitometric analysis appears in Results. GGA, 400 mg/kg, was orally administered twice at 18 and 2 h before injury.