Skip to main content
. Author manuscript; available in PMC: 2021 Mar 29.
Published in final edited form as: Kidney Int. 2011 Jan 26;79(8):861–870. doi: 10.1038/ki.2010.527

Figure 6 |. Effect of heat shock protein 70 (Hsp70) expression on Akt-glycogen synthase kinase 3β (GSK3β) activity after renal ischemia and effect of stress on Bax serine phosphorylation.

Figure 6 |

(a) Steady-state content of active (p-ser473) and total Akt, inactive (ser9) and total GSK3β, and β-actin in renal cortical homogenates obtained from Hsp70+/+, Hsp70+/−, and Hsp70−/− mice at baseline, immediately after 25 min of bilateral renal pedicle occlusion (RPO), or after 30, 60, or 120 min of reperfusion. (b) Densitometric assessment of active Akt and inactive GSK3β at 30 min after bilateral RPO; *P<0.05 vs sham control at baseline; n = 6 separate experiments. (c) Effect of ATP depletion on 32[P] incorporation into a synthetic Bax peptide containing the Akt-specific phosphorylation site (ser184); *P<0.05 vs baseline; n = 3.