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Sulforhamnose is a derepressor of the transcriptional repressor CsqR. (a) PAGE gel shift assay showing effect of SQ and SR on binding of CsqR to a DNA probe of the yihUV intergenic region. Effector concentrations, lanes 1–8: 0, 0, 5, 10, 25, 50, 75, 100 mM. DNA was stained with GelRed and imaged. (b) Representative AFM observations of the effect of SQ and SR on binding of CsqR to yihUV intergenic DNA probe. All images cover an area of 250 × 250 nm.
