Skip to main content
. Author manuscript; available in PMC: 2021 Mar 29.
Published in final edited form as: J Pathol. 2020 Sep 26;252(4):371–383. doi: 10.1002/path.5531

Figure 4.

Figure 4.

CRAMP KO mice had increased hepatic oxidative stress by alcohol. (A) Xanthine oxidase (XO) activity in the serum and liver lysates of alcohol-fed mice. (B) Immunoblotting and quantification of CYP2E1 protein. The bands are composite images of selected bands, and the corresponding β-actin controls are shown. (C) Liver ROS production and quantification by DHE staining. Original magnification: 100×. Data are expressed as mean SEM (n = 8–10). (D) Validation of knockdown efficiency by siRNA-Camp transfection in desferrioxamine (DFO)-treated mouse hepatocyte Hepa1–6 cells. (E) Ethanol-induced UA production in Hepa1–6 culture supernatant. siRNA-NT: non-targeting siRNA control; siRNA-Camp: Camp-targeting siRNA; EtOH: ethanol (400 mM for 4 h). Data are expressed as mean SEM (n = 4–6).