Figure 2.

General approach of polymer-based methods for tissue regeneration typically involving application of a composite of biodegradable natural or synthetic polymeric matrices, growth factors, and seeding cells to the damaged tissue site. In step (1) growth factors and cells are seeded in polymer or cells are seeded on growth factor conjugated polymer; in (2) nutrients, oxygen, and appropriate physiological support are supplied to CPGFC in vitro for conditioning before application in vivo or may be applied directly without conditioning; and in (3) site of the tissue injury is dressed with bio-functional CPGFC composite materials.