Skip to main content
. 2021 Mar 11;11:637878. doi: 10.3389/fonc.2021.637878

Figure 3.

Figure 3

KIF5B and CYP24A1 expression is inhibited by the miR-1278 mimic in CRC cells. (A) The miR-1278 mimic was transfected into three CRC cell lines (HT29, HCT116 and SW620), and RNA-seq was performed to compare whole gene expression variations between the mimic group and the control group. (B) In total, 1,612 genes, 2,606 genes, and 903 genes were screened at an absolute fold change value > 2 in HT29, HCT116 and SW620 cells, respectively. Only 54 genes consistently differed among the three cell lines. (C) Among them, two genes, KIF5B and CYP24A1, were selected for further analysis. The mRNA expression of KIF5B and CYP24A1 was verified by qRT-PCR in HT29 and HCT116 cells. (D) The protein expression of KIF5B and CYP24A1 was determined by Western blotting, indicating that the miR-1278 mimic markedly decreased the protein expression of KIF5B and CYP24A1 in HT29 and HCT116 cells. (E) The predicted consequential pairing of miR-1278 and the 3′UTR of KIF5B or CYP24A1 was determined. (F) The luciferase reporter assay showed that miR-1278 directly binds with the KIF5B 3′UTR and CYP24A1 3′UTR. NC, negative control; Mimic, miR-1278 mimic; WT, wild type; Mut, mutated; *P < 0.05, **P < 0.01, ***P < 0.001, N.S, no significance.