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. 2021 Mar 15;9:647702. doi: 10.3389/fcell.2021.647702

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Copyright © 2021 Chen, Liang, Yi, Fan, Chen, Zhang and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of YTHDF1 suppresses cell growth, migration and invasion in vitro. (A) Western blot analysis for YTHDF1 expression in GES-1 and GC cell lines (AGS, BGC-823, SGC-7901, MKN-28, and HGC-27). (B) Western blot analysis for YTHDF1 expression in AGS and BGC-823 cells infected with two independent shRNAs targeting YTHDF1 (sh-YTHDF1#1/2) or a control shRNA (sh-NC). (C) Colony formation assays of AGS and BGC-823 cells described in (B). (D) CCK8 assays were performed to determine cell growth in YTHDF1 deficient AGS and BGC-823 cells as described in (B). (E,F) Knockdown of YTHDF1 decreased the abilities of migration and invasion of AGS and BGC-823 cells. Scale bar, 50 μm. (G) Cell apoptosis analysis was used to compare down-expression of YTHDF1 (sh-YTHDF1#1/2) with the sh-NC group in BGC-823 and AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001; ns, no significance.