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. 2021 Mar 9;11:602712. doi: 10.3389/fonc.2021.602712

Figure 1.

Figure 1

rhIL6 intervention before lobaplatin treatment of osteosarcoma cells increased cell viability and reduced SaOS-2 and SOSP-9607 cell apoptosis. (A) Cell viability measured by CCK-8 assay in the CG, rhIL-6G, LG, and rhILG. Experiments were performed with at least three biological replicates, and a p ≤ 0.05 was considered statistically significant. (B) Cells in each group were stained with annexin V and PI and measured by flow cytometry. Early and late apoptotic cells were demonstrated as annexin V+/PI– (early apoptosis) and annexin V+/PI+ (late apoptosis), respectively. (C) Morphological differences in osteosarcoma cells in the CG, rhIL-6G, LG, and rhILG were observed by bright-field microscopy (×200), scale bar = 100 μm. (D) Morphological differences in osteosarcoma cell nuclei were detected by Hoechst 33342 staining and observed by fluorescence microscopy (×200), scale bar = 100 μm. *p < 0.05; **p < 0.01.