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. 2021 Mar 9;11:637298. doi: 10.3389/fonc.2021.637298

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Copyright © 2021 Sanches, Song, Zhang, Cui, Yabasin, Ntim, Li, He, Zhang, Mao, Lu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The downregulation of KDM2B regulates the cell stemness in CRC in CRC cell lines. Representative images of adherent cells and the tumor sphere. (A) The assessment of the expression level of KDM2B protein in adherent cells and the tumor sphere was analyzed by Western blotting. (B) Tumor sphere formation of HT-29 and DLD-1 cells. Cells were treated with siKDM2B (#1 and #2) or negative control (NC) for 7 days, and expansion of the tumor spheres were analyzed at 20 × and 40 × magnification under a microscope (bar = 50 μm; magnification, 200 × and 400 ×). (C) The cluster bar chart of the number and size of spheres formed. (D,E) The effect of KDM2B on CRC cell stem-like markers. CD44, CD133, ALDH-1, and KDM2B levels were analyzed by Western blotting. Data are expressed as Mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. control.