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. 2021 Mar 29;16:226. doi: 10.1186/s13018-021-02372-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The expression of Jagged1 in and osteosarcoma specimens and cells. a Representative pictures of strong positive (+++), moderate positive (++), weak positive (+), and negative (−) staining of Jagged1 immunohistochemistry in osteosarcoma specimens. Scale bar = 50 μm. b Relative expression of Jagged1 mRNA in F4 osteosarcoma cells with low metastasis and F5M2 osteosarcoma cells with high metastasis detected by real-time quotative RT-PCR. **P<0.01. c The expression of Jagged1 protein in F4 osteosarcoma cells with low metastasis and F5M2 osteosarcoma cells with high metastasis detected by Western blotting