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. 2021 Mar 15;9:647527. doi: 10.3389/fcell.2021.647527

Figure 5.

Figure 5

SENP3-EIF4A1 functions as a sponge for miR-195-5p. (A–C) SENP3-EIF4A1 expression in TNBC and luminal-type breast cancer from TCGA BRCA database (A), clinical samples (B), and cell lines (C). (D) Schematic illustration of SENP3-EIF4A1 luciferase reporter vectors. (E) The relative luciferase activities were detected in SUM1315 cells after transfection. (F) Anti-AGO2 RIP was executed in transfected SUM1315 cells and agarose gel electrophoresis, qRT-PCR to detect expression. (G,H) Expression correlation between SENP3-EIF4A1 and miR-195-5p (G) or CCNE1 (H) in 30 TNBC samples. (I,J) SENP3-EIF4A1 (I) and miR-195-5p (J) expression shown by qRT-PCR after transfecting si-NC, si SENP3-EIF4A1, pl-Ctrl, and pl-SENP3-EIF4A1 in cells. (K) mRNA and protein level of CCNE1 expression of transfected cells. Data were presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.