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. 2021 Mar 15;9:647527. doi: 10.3389/fcell.2021.647527

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Copyright © 2021 Chen, Miao, Si, Qin, Zhang, Chu, Li, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Methylation levels of miR-195-5p promoter region in TNBC. (A–C) TCGA BRCA database showed DNA methylation of miR-195-5p in TNBC samples as compared with luminal-type breast cancer or normal breast samples (A), miR-195-5p DNA methylation level and miR-195-5p expression correlation in TNBC (n = 82) (B), miR-195-5p DNA methylation level, and CCNE1 expression correlation in TNBC (n = 82) (C). (D) qRT-PCR of miR-195-5p expression in MDA-MB-231 cells with or without 5-AzaDc treatment. (E,F) mRNA (E) and protein (F) level of CCNE1 expression in MDA-MB-231 cells with or without treatment. *p < 0.05; **p < 0.01; ****p < 0.0001.